Zinc-finger antiviral protein (ZAP) is a restriction

Aug 31, 2020 · The zinc-finger RNA binding protein ZAP was identified as a candidate restriction factor for MVA in an unbiased high-throughput human genome-wide RNAi screen in human A549 cells. The anti-viral role of endogenous ZAP was confimed by demonstrating that MVA replication was enhanced in four human cell lines by treating them with additional siRNAs

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Addgene Zinc Finger Consortium OPEN Reagents

For your Materials and Methods section "The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section Oligomerized pool engineering (OPEN) an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK.

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Engineered zinc finger nickases induce homology-directed

For in vitro protein expression experiments, we constructed vectors derived from pMLM290 and pMLM292 [Addgene plasmids 21872 and 21873, respectively ( 14)]. Zinc finger domains in the ZFNs HX735, VF2468 and VF2471 have been described previously ( 14)

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A previously unknown zinc finger protein, DST, regulates

The zinc finger motif is thought to recognize and bind its target DNA sequence, but has never been shown to be required for transcriptional activity (Wolfe et al. 2000; Sakamoto et al. 2004; Lin et al. 2007). However, here we surprisingly found that the single zinc finger motif of DST is required not only for its DNA-binding activity but also

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Pfam Family zf-C2H2 (PF00096)

A zinc finger is a small protein structural motif that is characterized by the coordination of one or more zinc ions (Zn 2+) in order to stabilize the fold.Originally coined to describe the finger-like appearance of a hypothesized structure from Xenopus laevis transcription factor IIIA, the zinc finger name has now come to encompass a wide variety of differing protein structures.

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ZiFiT

Digest 3-5 µg of pSQT1313 (available via Addgene) with BsmBI (NEB) overnight at 55°C. 2. Heat inactivate for 20 minutes at 80°C. Zinc Fingers (ZFs) A ZF is a protein motif consisting of two beta strands and an alpha helix, stabilized by coordination of a zinc ion by pairs of conserved cysteine and histidine residues. Residues1 to 6

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CRISPR 101 Homology Directed Repair Addgene

Zinc finger nucleases (ZFNs), TAL effector nucleases (TALENs)m and CRISPR can all direct a nuclease to cause a specific DSB. The ease of creating guides, the speed of the system, and its targeting versatility have not just reinvigorated genome engineering, but have really revolutionized the field.

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Zinc Finger Nucleases Targeting the Human Papillomavirus

Zinc finger nuclease (ZFN) is an artificial endonuclease which is engineered by fusing DNA-binding zinc finger proteins to the FokI DNA-cleavage domain . Cleavage is induced when two custom-designed ZFNs dimerize upon binding DNA to form an active FokI nuclease. By cleaving specific target DNA, ZFNs create double-strand breaks (DSB) that are

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Protein Delivery Using Cys2His2 Zinc-Finger Domains ACS

The development of new methods for delivering proteins into cells is a central challenge for advancing both basic research and therapeutic applications. We previously reported that zinc-finger nuclease proteins are intrinsically cell-permeable due to the cell-penetrating activity of the Cys2His2 zinc-finger domain. Here, we demonstrate that genetically fused zinc-finger motifs can transport

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Zinc-finger nuclease-mediated targeted insertion of

Jul 03, 2012 · To understand complex biological systems, such as the development of multicellular organisms, it is important to characterize the gene expression dynamics. However, there is currently no universal technique for targeted insertion of reporter genes and quantitative imaging in multicellular model systems. Recently, genome editing using zinc-finger nucleases (ZFNs) has been reported in several

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EGR1 Gene GeneCards EGR1 Protein EGR1 Antibody

EGR1 (Early Growth Response 1) is a Protein Coding gene. Diseases associated with EGR1 include Ischemia and Embryonal Carcinoma.Among its related pathways are Neuroscience and Preimplantation Embryo.Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and transcription factor binding.

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() Zinc Finger Targeter (ZiFiT) An engineered zinc

Zinc Finger Modules and Binding Sites. (a) A canonical zinc finger module consists of two anti-parallel beta strands and an alpha helix, with a zinc ion coordinated by the conserved cysteine (C

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Addgene Wikipedia

Addgene collaborates with institutes and consortia to curate plasmid collections for specific purposes. Examples of these collaborations include special collections from the Structural Genomics Consortium, Zinc Finger Consortium, the Cell Migration Consortium, the KLF

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Zinc-finger nuclease-mediated targeted insertion of

Jul 03, 2012 · To understand complex biological systems, such as the development of multicellular organisms, it is important to characterize the gene expression dynamics. However, there is currently no universal technique for targeted insertion of reporter genes and quantitative imaging in multicellular model systems. Recently, genome editing using zinc-finger nucleases (ZFNs) has been reported in several

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Has anyone of you successfully used the Addgene Zinc

I have seen a few papers that suggest that solubility of some zinc binding/RING finger-containing proteins may be increased with the addition of ZnCl2 (10-100uM), but I am open to any additional

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Addgene Wikipedia

Addgene collaborates with institutes and consortia to curate plasmid collections for specific purposes. Examples of these collaborations include special collections from the Structural Genomics Consortium, Zinc Finger Consortium, the Cell Migration Consortium, the KLF

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() Efficient Methods for Targeted Mutagenesis in

Efficient Methods for Targeted Mutagenesis in Zebrafish Using Zinc-Finger Nucleases Data from Targeting of Nine Genes Using CompoZr or CoDA ZFNs Raman Sood1*, Blake Carrington1., Kevin Bishop1., MaryPat Jones2, Alberto Rissone3, Fabio Candotti3, Settara C. Chandrasekharappa2, Paul Liu4 1 Zebrafish Core Facility, Genetics and Molecular Biology Branch, National Human Genome

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CRISPR 101 Validating Your Genome Edit Addgene

The techniques described in this post are not CRISPR-specific and can also be used for assessing genome edits created by TALEN or Zinc Finger Nucleases. Regardless of what method you use, validating your edit is time well spent as you prepare for your future experiments.

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Addgene Zinc Finger Consortium Nuclease Expression Vectors

Zinc Finger Consortium Nuclease Expression Vectors. This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created using ZFC reagents for either OPEN or modular assembly can be easily transferred in-frame to

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Wolfe-Lawson ZFN modular assembly kit Addgene

[email protected] Wolfe-Lawson ZFN modular assembly kit Description Zinc-finger nucleases (ZFNs) allow targeted gene inactivation in a wide range of model organisms. We provided an archive of 81 clones containing 27 different zinc-finger modules at finger 1, 2 and

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A guide to genome engineering with programmable nucleases

Apr 02, 2014 · An unbiased genome-wide analysis of zinc-finger nuclease specificity. Nature Biotech. 29, 816823 (2011). References 117 and 119 demonstrate two different methods to identify off-target sites

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SNAI2 Zinc finger protein SNAI2 Homo sapiens (Human

Interacts (via zinc fingers) with KPNA2, KPNB1, and TNPO1. May interact (via zinc fingers) with IPO7. By similarity 1 Publication,,,, Binary interactions i. Show more details Hide details. O43623. With #Exp. IntAct; CABP2 [Q9NPB3] 3 EBI-9876238,EBI-12011224 CSNK2A1 [P68400]

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A previously unknown zinc finger protein, DST, regulates

The zinc finger motif is thought to recognize and bind its target DNA sequence, but has never been shown to be required for transcriptional activity (Wolfe et al. 2000; Sakamoto et al. 2004; Lin et al. 2007). However, here we surprisingly found that the single zinc finger motif of DST is required not only for its DNA-binding activity but also

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Accelerating research through reagent repositories the

Nov 20, 2015 · Addgene is a nonprofit plasmid repository that was founded in 2004 to overcome the obstacles with plasmid sharing. Shortly after its conception, Addgene launched a fruitful collaboration with the Zinc Finger Consortium, which was also in its infancy.

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Zinc Finger Nucleases Point to More Precise Creation of

Jun 10, 2011 · Zinc Finger Nuclease (ZFN) technology burst onto the research scene approximately a decade ago, inspiring new visions of genetic modification possibilities. The discovery of the use of ZFNs for deleting genes from and inserting genes into organisms was an

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Addgene Find link

Zinc finger nuclease (4,640 words) exact match in snippet view article find links to article PMC 2502069. PMID 18500337. Zinc finger selector Zinc Finger Consortium website Zinc Finger Consortium materials from Addgene A commercial supplier of ZFNs

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A History of Genome Engineering in Popular Culture Addgene

Zinc finger nucleases. Zinc finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain (FokI). Zinc finger nucleases were first designed in 2005. The DNA-binding domain can be engineered to target specific DNA sequences while FokI then induces a double strand break.

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CRISPR 101 Homology Directed Repair Addgene

Zinc finger nucleases (ZFNs), TAL effector nucleases (TALENs)m and CRISPR can all direct a nuclease to cause a specific DSB. The ease of creating guides, the speed of the system, and its targeting versatility have not just reinvigorated genome engineering, but have really revolutionized the field.

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Genome Editing with CRISPRs, TALENs and ZFNs Biocompare

Aug 27, 2013 · Though zinc fingers work, they're relatively expensive and finicky to make, and researchers generally have to order them from Sigma Aldrich (though home-brew designs also exist, for instance at Addgene.org). Recently, however, a similar,

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387609 Gene ResultZhx2 zinc fingers and homeoboxes 2

Gene ID 387609, updated on 3-Oct-2020. Summary Other designations. zinc fingers and homeoboxes protein 2, AFP regulator 1, alpha fetoprotein regulation 1, adult, alpha-fetoprotein regulator 1, regulator of AFP, zinc finger and homeodomain protein 2. GeneRIFs Gene References Into Functions. On the high-fat chow, BALB/cJ mice with a mutation encoding the transcription factor Zinc fingers and

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